Also called Microhematocrit Tubes, taken from the Merriam Webster Dictionary definition, the word “Microhematocrit” means: “A procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin.”
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Contents

What is the purpose of a Microhematocrit tube?

Kimble™ micro-hematocrit tubes are used to measure the volume percentage of red blood cells in blood.

How much does a Microhematocrit tube hold?

The ratio of the volume of packed red cells to the total blood volume is called the hematocrit. Heparinized blood is transferred from the microcentrifuge tube (often called eppendorf tube) to a microhematocrit capillary. The tube is filled to at about 3/4 capacity by capillary action.

How long is the plug of Microhematocrit tube?

For the microhematocrit method, after filling the blood in a capillary tube, the two ends of the tube (commonly 75 mm long, 1 mm diameter) are sealed with clay sealant or heat.

What is the difference between a Microhematocrit and a regular hematocrit?

Microhematocrit method is a gold standard method for hematocrit determination but it associates many problems that may lead to inaccurate and imprecise measurements. Spun hematocrit is 1% to 3% higher than the hematocrit from automated instrument due to plasma that is trapped in erythrocytes.

What are advantages of the Microhematocrit test?

What are advantages of the microhematocrit test? The microhematocrit only requires a small amount of blood and it is a CLIA-waived test. Name a condition that could cause a decreased hematocrit value. A decreased hematocrit value can indicate a condition such as anemia or the presence of bleeding in a patient.

What acts as an anticoagulant on the surface of Microhematocrit tubes?

Glass Micro-Hematocrit Capillary Tubes The tubes are available plain or with an anticoagulant (sodium or ammonium heparin) that keeps the blood from clotting.

How full should a hematocrit tube be filled?

Hold the tube at an angle and introduce the microhaematocrit (capillary) tube. Allow blood to track up the tube. Continue until the tube is about 3/4 full. Put your index finger over the top of the capillary tube before removing it from the sample or blood will leak!

What is the normal hematocrit?

Generally, a normal range is considered to be: For men, 38.3 to 48.6 percent. For women, 35.5 to 44.9 percent.

How do you run a Microhematocrit?

  1. Fill two capillary tubes approximately 2/3 to 3/4 full with the well-mixed blood sample.
  2. Seal the dry end of the capillary tube by placing it into the sealing clay at a 90o angle.
  3. Place the capillary tubes in the microhematocrit centrifuge with the sealed end toward the periphery. …
  4. Centrifuge for five minutes.
How is Microhematocrit measured?

Microhematocrit tube after sedimentation. The hematocrit is a ratio of the packed cells to total volume. Example: If the column of packed red cells measures 20 mm and the whole blood column measures 50 mm, the hematocrit is 20/50 = 0.4 or (0.4 × 100%) = 40%.

What happens if Haematocrit is low?

A low hematocrit level means the are too few red blood cells in the body. In these cases, a person may experience symptoms that signal anemia. Common symptoms include fatigue, weakness, and low energy. If a person has too many red blood cells, they have a high hematocrit level.

What are the precautions to be observed when performing a Microhematocrit?

What safety precautions should be observed when performing a microhematocrit? Observe Standard precautions; use plastic or Mylar-coated self-sealing tubes; close centrifuge lids securely; never open centrifuge until rotor has stopped; dispose of all sharps in appropriate containers.

Is it possible to have a low hemoglobin but high hematocrit level?

Just as low hemoglobin levels signify anemia, a person with a low hematocrit percentage is also anemic. High hematocrit percentages are seen in the same populations that may have high hemoglobin levels.

What is the purpose of the hemolysis applicator?

What is the purpose of the hemolysis applicator? It breaks down red blood cells and releases hemoglobin to the solution.

When would you use a heparinized capillary tube for performing Microhematocrit?

Capillary tubes for microhaematocrits are used for the centrifugation of blood samples. Heparinized capillaries should be used when the samples are not immediately sealed with wax and centrifuged.

Why would your Rdw be high?

High results If your RDW is too high, it could be an indication of a nutrient deficiency, such as a deficiency of iron, folate, or vitamin B-12. These results could also indicate macrocytic anemia, when your body doesn’t produce enough normal red blood cells, and the cells it does produce are larger than normal.

When measuring the Microhematocrit what results should I read?

After centrifugation, the hematocrit is read by measuring from the bottom of the red cell column to the top of the buffy coat.

What are the two possible causes of a low Microhematocrit value?

  • Anemia.
  • Nutritional deficiency of iron, vitamin B-12, or folate.
  • Kidney disease.
  • A bone marrow disease.
  • Certain cancers such as leukemia, lymphoma, or multiple myeloma.
What is the anticoagulant of choice in the Microhematocrit procedure?

The anticoagulant of choice is 1.1% sodium oxalate in distilled water. Haden’s modification. This method used a calibrated tube.

What are the different Microhematocrit tube types?

  • Fisherbrand™ Microhematocrit Capillary Tubes. …
  • EKF ClearCRIT™ Capillary Tubes. …
  • Drummond™ Hematocrit Tubes. …
  • HemoCue America StatSpin™ SafeCrit™ Plastic Microhematocrit Tubes. …
  • Greiner Bio-One Lithium Heparin Tubes. …
  • Drummond™ Hemato-Clad™ Mylar™-Wrapped Hematocrit Tubes.
What color tipped capillary tube is used with anticoagulated blood?

Gray-top tube: Contains sodium fluoride (a preservative) and potassium oxalate (an anticoagulant). Use: Sodium fluoride whole blood or plasma.

How is hematocrit formed?

An estimated hematocrit as a percentage may be derived by tripling the hemoglobin concentration in g/dL and dropping the units. The packed cell volume (PCV) can be determined by centrifuging EDTA-treated or heparinized blood in a capillary tube (also known as a microhematocrit tube) at 10,000 RPM for five minutes.

What color tube is HCT?

Specimen required: EDTA lavender-top tube or microcollection tube.

What happens if your hematocrit is too high?

If you’ve taken a hematocrit test and hematocrit is high, this means that you have more red blood cells than what’s considered to be healthy. High hematocrit levels could indicate underlying medical conditions like: Dehydration. Carbon monoxide poisoning.

What does it mean if hematocrit is high?

If your hematocrit is high, it means that you have more red blood cells than is considered healthy. High hematocrit may be caused by [4:1]: Heart disease. Dehydration.

What diseases cause low hematocrit?

  • Blood loss.
  • Leukemia or other bone marrow problems.
  • Iron and vitamin deficiency, including folate, vitamin B12, and vitamin B6.
  • Too much water in the body.
  • Kidney disease.
  • Thyroid abnormality.
  • Immune destruction of red blood cells.
Which test is a good indicator for anemia?

Often, the first test used to diagnose anemia is a complete blood count (CBC). The CBC measures many parts of your blood. The test checks your hemoglobin and hematocrit (hee-MAT-oh-crit) levels. Hemoglobin is the iron-rich protein in red blood cells that carries oxygen to the body.

What area of the body does a hematologist study?

Hematology is the study of blood and blood disorders. Hematologists and hematopathologists are highly trained healthcare providers who specialize in diseases of the blood and blood components. These include blood and bone marrow cells.

Why would hemoglobin and hematocrit be low?

Possible causes of low Hgb include: lack of iron in your diet, which makes it harder for your bone marrow to produce Hgb. lack of folate or vitamin B-12, which can lead to your body producing fewer red blood cells than are needed. severe blood loss after surgery or a major injury.

What should your MCV level be?

An MCV test measures the size and volume of red blood cells. A normal MCV range is roughly 80–100 fl . If someone’s MCV level is below 80 fl, they will likely develop or have microcytic anemia.

What hematocrit level is considered anemic?

Normal hematocrit levels vary based on age and race.. In adults, normal levels for men range from 41%-50%. For women, the normal range is slightly lower: 36%-44%. A hematocrit level below the normal range, meaning the person has too few red blood cells, is called anemia.

How do you treat low hematocrit?

Most people are not treated with medications or procedures if the hematocrit is only slightly above or below the normal levels. Some patients with very low hematocrits may require intravenous iron, transfusions or medications to stimulate the production of red cells by the bone marrow.

How can I raise my hematocrit?

What can I do to increase my low hematocrit? Increasing the consumption of red meat (liver in particular), fish and shellfish (oysters, clams, shrimp, and scallops), dried fruit (apricots, prunes, and peaches), green leafy vegetables, beans, iron fortified breads and cereals, all rich in iron, may help.

What are two conditions that cause polycythemia?

  • Hypoxia from long standing (chronic) lung disease and smoking are common causes of polycythemia. …
  • Chronic carbon monoxide (CO) exposure can also be a risk factor for polycythemia.
What does the blue ring around the Microhematocrit tube mean?

Capillary tubes with a blue ring do not have an anticoagulant and do not need to be mixed. Capillary tubes with a red ring contain the anticoagulant heparin, which prevents blood from clotting. The red ringed tubes must be inverted several times to allow the blood to mix with the heparin.

Will a dermal puncture cause pain to the patient?

However, if the puncture is not performed correctly, or an approved site is not used, the puncture may cause more discomfort, or even injury to the patient.

Where should you collect a dermal sample from a toddler?

The heel is the recommended site for collection of skin puncture specimens on infants less than 1 year old or not walking. However, it is important that the puncture be performed in an area of the heel where there is little risk of puncturing the bone. Skin punctures must not be performed on fingers of newborns.